Practical genotyping by single-nucleotide primer extension
نویسندگان
چکیده
منابع مشابه
Genotyping single nucleotide polymorphisms in barley by tetra-primer ARMS-PCR.
Single nucleotide polymorphisms (SNPs) are the most abundant form of DNA polymorphism. These polymorphisms can be used in plants as simple genetic markers for many breeding applications, for population studies, and for germplasm fingerprinting. The great increase in the available DNA sequences in the databases has made it possible to identify SNPs by "database mining", and the single most impor...
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BACKGROUND Genotyping of N-acetyltransferase-2 (NAT2) is useful in predicting the risk for toxicity of NAT2 substrates. Current methods cannot detect the 7 most important single-nucleotide variations in NAT2 simultaneously in 1 tube. METHODS We developed an assay that uses allele-specific primer extension (ASPE) and microsphere hybridization for the simultaneous detection of 7 single-nucleoti...
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The resistance of Plasmodium falciparum to some antimalarial drugs is linked to single-nucleotide polymorphisms (SNPs). Currently, there are no methods for the identification of resistant parasites that are sufficiently simple, cheap, and fast enough to be performed at point-of-care, i.e., in local hospitals where drugs are prescribed. Primer extension methods (PEXT) were developed to identify ...
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en, depending on the rotisserie motor kit you choose. Widely available power bars can serve as an on/off switch for motors not equipped with their own. Membranes are inserted into the 50 mL tubes. The surface carrying the nucleic acids or proteins should face inwards. Membranes should be moist to prevent breakage and air bubble formation from surface tension. To start the operation, reagent sol...
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The reactivity of an RNA ribose hydroxyl is shown to be exquisitely sensitive to local nucleotide flexibility because a conformationally constrained adjacent 3'-phosphodiester inhibits formation of the deprotonated, nucleophilic oxyanion form of the 2'-hydroxyl group. Reaction with an appropriate electrophile, N-methylisatoic anhydride, to form a 2'-O-adduct thus can be used to monitor local st...
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ژورنال
عنوان ژورنال: Biology Methods and Protocols
سال: 2020
ISSN: 2396-8923
DOI: 10.1093/biomethods/bpaa002